VanGuard 1300 Series, Microscope 411, Brightfield & Phase Contrast Explained

June 11, 2009 Vol. 1, No. 2

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VanGuard 1300 Series Clinical Microscopes are a great match for use in medical and veterinary clinics, universities, and bio research laboratories. These four models meet a variety of application needs.

Model	Head	Type	Objectives	List Price
1321BRi	Binocular	Brightfield	4X,10X,40X,100X	$1,175
1331BRi	Trinocular	Brightfield	4X,10X,40X,100X	$1,250
1323PHi	Binocular	Phase Contrast, Brightfield, & Darkfield	10X,20X,40X,100X	$1,675
1333PHi	Trinocular	Phase Contrast, Brightfield, & Darkfield	10X,20X,40X,100X	$1,755

Features
Infinity-corrected optics • Large 20mm eyepiece field diameter • High eyepoint eyepieces with soft rubber eyecups • Plan achromatic objectives produce an image in which nearly 100% of the field is in focus simultaneously • Koehler illumination provides uniform brightness and eliminates glare • Brightfield and Darkfield stops on phase contrast condensers • Advanced ergonomics

Model 1333PHi has a trinocular port, ideal for outfitting a camera system. Also take a look at the base of the microscope; the low focusing controls alleviate wrist strain for the user.

Microscope Girl
Microscope 411
Whether you know a lot about microscopes or haven't touched one since sophomore biology class, we can help you choose a VanGuard Microscopes for your application.

Call and pick our brains: (800) 423-8842. Folks consult us regularly about microscopes.

What’s the difference between Brightfield and Phase Contrast?

Brightfield refers to the traditional transmitted light microscopy where the light is directed through the sample and then to the user's eye. Contrast in the sample is obtained by using a number of different techniques including staining and light intensity adjustment. The sample is seen as a darkened or colored structure against a white (bright) background or field.

Phase contrast is a lighting configuration designed to induce contrast into a sample without staining. This makes it possible to view the colorless components of living cells and bacteria. Phase contrast uses a series of "light baffles" to take advantage of refractive differences in the sample structures. It's worth noting that all the phase contrast models in the 1300 series are capable of phase contrast, brightfield, and darkfield illumination.

(800) 423-8842